Poster Presentation 16th Asian Conference on Transcription 2019

CHD Family have Distinct Translocation Dynamics on Nucleosome Complex (1159)

Chanshin Kang 1 , Eunhye Lee 2 , Ji-Joon Song 2 , Sungchul Hohng 1
  1. Seoul National University, Gwanak-Gu, SEOUL, South Korea
  2. Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea

ATPase remodelers translocate DNA which bound along nucleosome. The remodeling mechanism takes huge parts of a central dogma which might be affect to DNA replication or transcription. Among various ATPase remodelers, Chromodomain-helicase-DNA-binding protein(CHD) family has similar helicase and ATPase domains which relates to SNF2. We used a single-molecule fluorescence approach to observe quantitative kinematics of remodelers on nucleosome complex that 601 DNA is bound along histone octamers. According to Fluorescence Resonance Energy Transfer(FRET) pictures, we got traces of a distance DNA related to histone nucleosome when ATPase remodelers are applied in high time resolution. Focus on CHD7, it relocates DNA in large steps but the step is composed of 1-2 fundamental steps. Compare to CHD1 which is also CHD family, CHD7 has not only similarity in remodeling activity but also noticeable different fundamental step size aspect. Our results distinguish ATPase remodelers: CHD7, CHD1 and ACF/Snf2h.